Abstract:
The genotoxicity of aluminum-gallic acid was investigated using DNA repair deficient Escherichia coli PQ37. The organometal complex was described using spectroscopic characteristics compared to gallic acid and aluminum chloride hexahydrate. Genotoxic concentrations of aluminum-gallic acid were utilized to assess their antimicrobial properties against Escherichia coli ATCC 25922. In addition, the complex was also evaluated in terms of its prooxidant property and effect on cell surface hydrophobicity. The minimumgenotoxic concentration of aluminum-gallic acid against E. coli PQ37 was ≥0.031μmol*mL-1. Cell surface hydrophobicity was decreased at a concentration of ≥0.06 μmol*mL-1 although there is no significant difference among different concentrations (ρ<0.01). The proposed mechanisms of aluminum-gallic acid were oxidative stress based from the formation of malondialdehyde-thiobarbituric acid (MDA-TBA) adducts. Gallic acid decreases the prooxidant effect of aluminum in a dose-dependent fashion, suggesting that aluminum is the cause of MDA production, not the complex.Putrescine supplementation decreases the antimicrobial properties of aluminum-gallic acid.
